Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. They provide an optimized method for studying isozyme-specific CYP450-drug interactions, metabolism, and inhibition. CYP450 Screening Kits offer:
• Easy three-step procedure, "mix and read" format, reactions at room temperature or 37°C
• Superior fluorescent properties and kinetics compared to conventional fluorogenic probes
• High signal-to-background ratio, broad dynamic range
• Compatible with multiple assay formats from 96-well to 1536-well
Simple Mix and Read Kit Format
CYP450 Screening Kits include Substrate, Fluorescent Standard, reaction buffer, Regeneration System, NADP+, and CYP450 Plus Reagents. The CYP450 Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme (CYP2D6 in this case) and human cytochrome-P450 reductase. CYP450 Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.
