The Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features: • Sensitive-highly sensitive detection of firefly luciferase activity. • Stable-the signal output is stable for more than two hours, providing flexibility with regard to incubation time • Convenient-simple one-step, homogeneous protocol. • High-throughput-one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications • Compatibility-works well with a variety of common media containing 0-10% serum and phenol red. • Instrumentation-does not require a luminometer with injectors.
