Product Name
Neutrophil Gelatinase-Associated Lipid Carrier Protein (NGAL)Test Kit (Immunofluorescence Chromatography)
Purpose
To detect the NGAL in human plasma/serum/whole blood in vitro quantitatively.For Professional Use only.
Principles of Detection
The NGAL Test is a fluorescent lateral flow immunoassay. The test consists of: 1) a conjugate pad containing NGAL antibody conjugated with fluorescent microspheres and a control antibody (goat anti-chicken IgY antibody) conjugated with fluorescence microspheres, 2) a nitrocellulose membrane strip containing a test line (T line) and a control line (C line). The T line is pre-coated with anotherNGAL antibody, and the C line is pre-coated with a control line antibody (chicken IgY antibody).
NGAL concentration in specimen is positively correlated with the fluorescent density of the microspheres, after the measure and calculation of the quantitative fluorescence immunoassay analyzer, then the analyzer would calculate the concentration of NGAL according to the preset calibration curve in the machine and display the result, detection unit is ng/mL. C line should appear in any cases, which indicates the reaction system is effective.
Applicable instrument
Avid fluorescent immunoanalyzer model: AI-IF 600
Reference range
Reference value: 37-180ng/mL; The reference value is verified by referring to similar products on the market. Due to ethnic and regional differences, each laboratory can establish its own reference interval according to the actual situation.
