New coronavirus COVID-19 nucleic acid detection kit (fluorescence RT-PCR method) instruction manual
[Product name] Generic name: Novel coronavirus COVID-19 nucleic acid detection kit (fluorescence RT-PCR method)
[Packaging specifications] 25 servings/box & 50 servings/box [Intended use] This kit is used to detect the novel coronavirus (COVID-19) ORF1ab and N genes, and is used as an aid to the novel coronavirus (COVID-19) Diagnosis and epidemiological monitoring.
[Test Principle] The new type of coronavirus belongs to the beta genus of coronaviruses. Novel coronavirus pneumonia is an acute respiratory infectious disease, and the population is generally susceptible. The source of infection currently seen is mainly patients infected with the new coronavirus, and asymptomatic infections may also become the source of infection. Based on the current epidemiological survey, the incubation period is 1-14 days, mostly 3-7 days. Main manifestations are fever, dry cough, and fatigue. A small number of patients are accompanied by nasal congestion, runny nose, sore throat, myalgia and diarrhea. This kit uses TaqMan probe method real-time fluorescent PCR technology to design specific primers and probes for the conserved region sequences of the novel coronavirus (COVID-19) ORF 1ab and N genes. It is equipped with PCR reaction solution, and uses one-step fluorescent RT-PCR technology to perform in vitro amplification detection of novel coronavirus (COVID-19) RNA on a fluorescent quantitative PCR machine to achieve qualitative analysis of the detection results. In addition, the PCR detection system contains human endogenous internal standard primers and probes, which monitor the sample collection and extraction process by detecting whether the internal standard is normal, and avoid false negative results.
[Main components] Serial number composition 25 people/box 50 people/box 1 COVID-19 PCR reaction solution 425μL×1 tube 850μL×1 tube 2 COVID-19 enzyme mixture 75μL×1 tube 150μL×1 tube 3 COVID- 19 Negative control 100μL×1 tube 100μL×1 tube 4 COVID-19 positive control 100μL×1 tube 100μL×1 tube 5 Instructions 1 part 1 part Note: 1) Reagents of different batch numbers cannot be mixed or interchanged. 2) The negative control is ribozyme-free water, and the positive control is the in vitro transcribed RNA containing the target gene (ORF1ab, N gene, internal standard gene). [Storage conditions and expiry date] 1. The kit should be stored at -20±5℃, and the expiration date of the kit is tentatively scheduled for 6 months. 2. The production date and the expiration date are shown in the outer packaging. 3. The kit should not be frozen and thawed more than 5 times, and the reagent should be used within 1 month after opening [Applicable Instruments] This kit is suitable for ABI series, Bio-Rad series, Agilent StratageneMX Series, Roche LightCycler R480, Cepheid SmartCycler, Rotor-Gene series and other multi-channel real-time fluorescent quantitative PCR instruments
