Tetracyclines(TCs)
ELISA
Test
Kit
1.
Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Tetracyclines in
the sample. The coupling antigen s are pre-coated on the micro-well stripes. The Tetracyclines in
the sample and the coupling antigen s pre-coated on the micro-well stripes compete for the anti-
Tetracyclines antibody. After the addition of the enzyme conjugate, the TMB substrate is added for
coloration. The optical density (OD) value of the sample has a negative correlation with the
Tetracyclines in it. This value is compared to the standard curve and the Tetracyclines
concentration is subsequently obtained.
2.
Technical
specifications
Sensitivity:
0.05 ppb
Detection
limit
:
Chicken,duck, pork(meat or liver ), shrimp, fish
Tetracyclines............................................................................................. 1ppb
Minocycline............................................................................................... 1ppb
RoliTetracyclines .......................................................................................1ppb
Aureomycin...............................................................................................1ppb
Demethylchlor Tetracyclines..................................................................... 3ppb
Terramycin................................................................................................ 2ppb
Doxycycline...............................................................................................3ppb
Honey
Tetracyclines............................................................................................. 2ppb
Minocycline............................................................................................... 2ppb
RoliTetracyclines .......................................................................................2ppb
Aureomycin...............................................................................................2ppb
Demethylchlor Tetracyclines..................................................................... 6ppb
Terramycin................................................................................................ 4ppb
Doxycycline...............................................................................................6ppb
Cross-reaction
rate
:
Tetracyclines............................................................................................100%
Minocycline..............................................................................................125%
RoliTetracyclines ......................................................................................110%
Aureomycin..............................................................................................100%
Demethylchlor Tetracyclines......................................................................35%
Terramycin..............................................................
